ELISA Kits, a Prototype Multiplex Electrochemoluminescent Assay

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Comparison of Commercial ELISA Kits, a Prototype Multiplex Electrochemoluminescent Assay, and a Multiplex Bead-Based Immunoassay for Detecting a Urine-Based Bladder-Cancer-Associated Diagnostic Signature.

The ability to accurately measure multiple proteins simultaneously in a single assay has the potential to markedly improve the efficiency of clinical tests composed of multiple biomarkers. We investigated the diagnostic accuracy of the two multiplex protein array platforms for detecting a bladder-cancer-associated diagnostic signature in samples from a cohort of 80 subjects (40 with bladder cancer).
Banked urine samples collected from Kyoto and Nara Universities were compared to histologically determined bladder cancer.
  • The concentrations of the 10 proteins (A1AT; apolipoprotein E-APOE; angiogenin-ANG; carbonic anhydrase 9-CA9; interleukin 8-IL-8; matrix metalloproteinase 9-MMP-9; matrix metalloproteinase 10-MMP10; plasminogen activator inhibitor 1-PAI-1; syndecan-SDC1; and vascular endothelial growth factor-VEGF) were monitored using two prototype multiplex array platforms and an enzyme-linked immunosorbent assay (ELISA) according to the manufacturer’s technical specifications.
  • The range for detecting each biomarker was improved in the multiplex assays, even though the lower limit of quantification (LLOQ) was typically lower in the commercial ELISA kits. The area under the receiver operating characteristics (AUROC) of the prototype multiplex assays was reported to be 0.97 for the multiplex bead-based immunoassay (MBA) and 0.86 for the multiplex electrochemoluminescent assay (MEA).
  • The sensitivities and specificities for MBA were 0.93 and 0.95, respectively, and for MEA were 0.85 and 0.80, respectively. Accuracy, positive predictive values (PPV), and negative predictive values (NPV) for MBA were 0.94, 0.95, and 0.93, respectively, and for MEA were 0.83, 0.81, and 0.84, respectively. Based on these encouraging preliminary data, we believe that a multiplex protein array is a viable platform that can be utilized as an efficient and highly accurate tool to quantitate multiple proteins within biologic specimens.
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PSMB8 antibody

22848-100ul 100ul
EUR 390

PSMB8 antibody

70R-19589 50 ul
EUR 435
Description: Rabbit polyclonal PSMB8 antibody

PSMB8 antibody

70R-13550 100 ul
EUR 457
Description: Affinity purified Rabbit polyclonal PSMB8 antibody

PSMB8 Antibody

1-CSB-PA775839
  • EUR 317.00
  • EUR 244.00
  • 100ul
  • 50ul
  • Form: Liquid
  • Buffer: -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol Antigen affinity purification
Description: A polyclonal antibody against PSMB8. Recognizes PSMB8 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;ELISA:1:2000-1:5000, WB:1:500-1:2000, IHC:1:25-1:100

PSMB8 Antibody

1-CSB-PA116429
  • EUR 317.00
  • EUR 244.00
  • 100ul
  • 50ul
  • Form: Liquid
  • Buffer: -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol Antigen affinity purification
Description: A polyclonal antibody against PSMB8. Recognizes PSMB8 from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;ELISA:1:1000-1:2000, WB:1:200-1:1000, IHC:1:25-1:100

PSMB8 Antibody

1-CSB-PA018886GA01HU
  • EUR 597.00
  • EUR 333.00
  • 150ul
  • 50ul
  • Form: Liquid
  • Buffer: PBS with 0.1% Sodium Azide, 50% Glycerol, pH 7.3. -20℃, Avoid freeze / thaw cycles. Antigen Affinity purified
Description: A polyclonal antibody against PSMB8. Recognizes PSMB8 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF

PSMB8 Antibody

1-CSB-PA018886LA01HU
  • EUR 317.00
  • EUR 335.00
  • 100ul
  • 50ul
  • Form: Liquid
  • Buffer: Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against PSMB8. Recognizes PSMB8 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC; Recommended dilution: IHC:1:20-1:200

PSMB8 Conjugated Antibody

C43134 100ul
EUR 397

Anti-PSMB8 antibody

STJ29479 100 µl
EUR 277
Description: The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 3 (proteasome beta 5 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. Two alternative transcripts encoding two isoforms have been identified; both isoforms are processed to yield the same mature subunit.

Anti-PSMB8 antibody

STJ11100809 100 µl
EUR 413
Description: The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 3 (proteasome beta 5 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. Two alternative transcripts encoding two isoforms have been identified; both isoforms are processed to yield the same mature subunit.

Psmb8/ Rat Psmb8 ELISA Kit

ELI-15652r 96 Tests
EUR 886

PSMB8 siRNA

20-abx904317
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol
  • Shipped within 5-10 working days.

PSMB8 siRNA

20-abx930156
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol
  • Shipped within 5-10 working days.

PSMB8 siRNA

20-abx930157
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol
  • Shipped within 5-10 working days.

pOTB7-PSMB8

PVT18497 2 ug
EUR 231

PSMB8 Antibody, HRP conjugated

1-CSB-PA018886LB01HU
  • EUR 317.00
  • EUR 335.00
  • 100ul
  • 50ul
  • Form: Liquid
  • Buffer: Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against PSMB8. Recognizes PSMB8 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA

PSMB8 Antibody, FITC conjugated

1-CSB-PA018886LC01HU
  • EUR 317.00
  • EUR 335.00
  • 100ul
  • 50ul
  • Form: Liquid
  • Buffer: Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against PSMB8. Recognizes PSMB8 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA

PSMB8 Antibody, Biotin conjugated

1-CSB-PA018886LD01HU
  • EUR 317.00
  • EUR 335.00
  • 100ul
  • 50ul
  • Form: Liquid
  • Buffer: Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against PSMB8. Recognizes PSMB8 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA

PSMB8 Blocking Peptide

BF0434-BP 1mg
EUR 195

PSMB8 Rabbit pAb

A7340-100ul 100 ul
EUR 308

PSMB8 Rabbit pAb

A7340-200ul 200 ul
EUR 459

PSMB8 Rabbit pAb

A7340-20ul 20 ul
EUR 183

PSMB8 Rabbit pAb

A7340-50ul 50 ul
EUR 223

anti-PSMB8 (1A5)

LF-MA30618 100 ul
EUR 527
Description: Mouse Monoclonal to PSMB8

Monoclonal PSMB8 Antibody, Clone: 1A5

AMM02864G 0.1ml
EUR 528
Description: A Monoclonal antibody against Human PSMB8. The antibodies are raised in Mouse and are from clone 1A5. This antibody is applicable in WB and IHC, ICC, E

Anti-PSMB8 / LMP7 Monoclonal Antibody

M02188-2 100ug
EUR 397
Description: Rabbit Monoclonal PSMB8 / LMP7 Antibody. Validated in IP, IF, WB and tested in Human, Mouse, Rat.

Rat PSMB8 shRNA Plasmid

20-abx984734
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg
  • Shipped within 15-20 working days.

Canine PSMB8 ELISA KIT

ELI-15651d 96 Tests
EUR 928

Human PSMB8 shRNA Plasmid

20-abx953843
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg
  • Shipped within 15-20 working days.

PSMB8 protein (His tag)

80R-2608 100 ug
EUR 322
Description: Purified recombinant PSMB8 protein

Mouse PSMB8 shRNA Plasmid

20-abx971337
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg
  • Shipped within 15-20 working days.

PSMB8 Recombinant Protein (Human)

RP085356 100 ug Ask for price

PSMB8 Recombinant Protein (Rat)

RP222629 100 ug Ask for price

PSMB8 Recombinant Protein (Mouse)

RP165287 100 ug Ask for price

Monoclonal PSMB8 Antibody (monoclonal) (M01), Clone: 1B3

AMM03954G 0.1mg
EUR 484
Description: A Monoclonal antibody against Human PSMB8 (monoclonal) (M01). The antibodies are raised in Mouse and are from clone 1B3. This antibody is applicable in WB, IHC and IF, IP, E

Proteasome Subunit Beta Type-8 (PSMB8) Antibody

20-abx114776
  • EUR 732.00
  • EUR 398.00
  • 150 ul
  • 50 ul
  • Shipped within 5-10 working days.

Proteasome Subunit Beta Type-8 (PSMB8) Antibody

20-abx141396
  • EUR 370.00
  • EUR 606.00
  • EUR 300.00
  • 100 ul
  • 200 ul
  • 50 ul
  • Shipped within 5-10 working days.

Proteasome Subunit Beta Type-8 (PSMB8) Antibody

abx011689-100ul 100 ul
EUR 411
  • Shipped within 5-10 working days.

Proteasome Subunit Beta Type-8 (PSMB8) Antibody

20-abx006453
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul
  • Shipped within 5-10 working days.

Proteasome Subunit Beta Type-8 (PSMB8) Antibody

abx028154-400ul 400 ul
EUR 523
  • Shipped within 5-10 working days.

Proteasome Subunit Beta Type-8 (PSMB8) Antibody

abx028154-80l 80 µl
EUR 286
  • Shipped within 5-10 working days.

Proteasome subunit beta type-8 (PSMB8) Antibody

20-abx211178
  • EUR 411.00
  • EUR 300.00
  • 100 ul
  • 50 ul
  • Shipped within 5-10 working days.

Proteasome subunit beta type-8 (PSMB8) Antibody

20-abx211851
  • EUR 411.00
  • EUR 300.00
  • 100 ul
  • 50 ul
  • Shipped within 5-10 working days.

[KO Validated] PSMB8 Rabbit pAb

A19933-100ul 100 ul
EUR 410

[KO Validated] PSMB8 Rabbit pAb

A19933-200ul 200 ul
EUR 571

[KO Validated] PSMB8 Rabbit pAb

A19933-20ul 20 ul
EUR 221

[KO Validated] PSMB8 Rabbit pAb

A19933-50ul 50 ul
EUR 287

PSMB8 ORF Vector (Human) (pORF)

ORF028453 1.0 ug DNA
EUR 405

Psmb8 ORF Vector (Rat) (pORF)

ORF074211 1.0 ug DNA
EUR 506

Psmb8 ORF Vector (Mouse) (pORF)

ORF055097 1.0 ug DNA
EUR 506

PSMB8 ELISA Kit (Human) (OKCD02695)

OKCD02695 96 Wells
EUR 792
Description: Description of target: The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides. Replacement of PSMB5 by PSMB8 increases the capacity of the immunoproteasome to cleave model peptides after hydrophobic and basic residues. Acts as a major component of interferon gamma-induced sensitivity. Plays a key role in apoptosis via the degradation of the apoptotic inhibitor MCL1. May be involved in the inflammatory response pathway. In cancer cells, substitution of isoform 1 (E2) by isoform 2 (E1) results in immunoproteasome deficiency. Required for the differentiation of preadipocytes into adipocytes.;Species reactivity: Human;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.117 ng/mL

PSMB8 ELISA Kit (Dog) (OKEH03975)

OKEH03975 96 Wells
EUR 844
Description: Description of target: ;Species reactivity: Dog;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.064 ng/mL

PSMB8 ELISA Kit (Human) (OKEH07770)

OKEH07770 96 Wells
EUR 1092
Description: Description of target: The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 3 (proteasome beta 5 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. Two alternative transcripts encoding two isoforms have been identified; both isoforms are processed to yield the same mature subunit.;Species reactivity: Human;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.06ng/mL

PSMB8 sgRNA CRISPR Lentivector set (Human)

K1740301 3 x 1.0 ug
EUR 339

Psmb8 sgRNA CRISPR Lentivector set (Mouse)

K4425601 3 x 1.0 ug
EUR 339

Psmb8 sgRNA CRISPR Lentivector set (Rat)

K6921501 3 x 1.0 ug
EUR 339

PSMB8 sgRNA CRISPR Lentivector (Human) (Target 1)

K1740302 1.0 ug DNA
EUR 154

PSMB8 sgRNA CRISPR Lentivector (Human) (Target 2)

K1740303 1.0 ug DNA
EUR 154

PSMB8 sgRNA CRISPR Lentivector (Human) (Target 3)

K1740304 1.0 ug DNA
EUR 154

Psmb8 sgRNA CRISPR Lentivector (Mouse) (Target 1)

K4425602 1.0 ug DNA
EUR 154

Psmb8 sgRNA CRISPR Lentivector (Mouse) (Target 2)

K4425603 1.0 ug DNA
EUR 154

Psmb8 sgRNA CRISPR Lentivector (Mouse) (Target 3)

K4425604 1.0 ug DNA
EUR 154

Human Proteasome subunit beta type-8 (PSMB8)

1-CSB-EP018886HU
  • EUR 380.00
  • EUR 214.00
  • EUR 1309.00
  • EUR 560.00
  • EUR 873.00
  • EUR 262.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
  • MW: 42.7 kDa
  • Buffer composition: Tris-based buffer with 50% glycerol.
Description: Recombinant Human Proteasome subunit beta type-8(PSMB8) expressed in E.coli

Psmb8 sgRNA CRISPR Lentivector (Rat) (Target 1)

K6921502 1.0 ug DNA
EUR 154

Psmb8 sgRNA CRISPR Lentivector (Rat) (Target 2)

K6921503 1.0 ug DNA
EUR 154

Psmb8 sgRNA CRISPR Lentivector (Rat) (Target 3)

K6921504 1.0 ug DNA
EUR 154

PSMB8 Protein Vector (Human) (pPB-C-His)

PV113810 500 ng
EUR 552

PSMB8 Protein Vector (Human) (pPB-N-His)

PV113811 500 ng
EUR 552

PSMB8 Protein Vector (Human) (pPM-C-HA)

PV113812 500 ng
EUR 552

PSMB8 Protein Vector (Human) (pPM-C-His)

PV113813 500 ng
EUR 552

Recombinant Human PSMB8 Protein, His, E.coli-1mg

QP13190-1mg 1mg
EUR 2757

Recombinant Human PSMB8 Protein, His, E.coli-20ug

QP13190-20ug 20ug
EUR 201

Recombinant Human PSMB8 Protein, His, E.coli-5ug

QP13190-5ug 5ug
EUR 155

PSMB8 Protein Vector (Rat) (pPB-C-His)

PV296842 500 ng
EUR 603

PSMB8 Protein Vector (Rat) (pPB-N-His)

PV296843 500 ng
EUR 603

PSMB8 Protein Vector (Rat) (pPM-C-HA)

PV296844 500 ng
EUR 603

PSMB8 Protein Vector (Rat) (pPM-C-His)

PV296845 500 ng
EUR 603

PSMB8 Protein Vector (Mouse) (pPB-C-His)

PV220386 500 ng
EUR 603

PSMB8 Protein Vector (Mouse) (pPB-N-His)

PV220387 500 ng
EUR 603

Comparison of Rapid Anti-HCV Multi-sure Kit with Gold Standard ELISA.

To compare the diagnostic yield of Multi-sure rapid HCV (hepatitis C virus) kit with ELISA.Comparative study.Pakistan Health Research Council, specialised research center for gastroenterology and hepatology, from August 2016 to January 2017.A modified rapid anti-HCV kit was compared with ELISA. This rapid kit is multi-parameter qualitative immune chromatographic kit for the in-vitro detection of antibodies to HCV in human blood.

  • Patients who came to PHRC, were tested using anti-HCV ELISA, and their test was run simultaneously on multi-sure HCV rapid kit were included in the study. Each positive and negative sample was included in this study. SPSS software was adapted for data analysis.A total of 420 samples were collected.
  • Among them, 255 (61%) were of male and 165 (39%) were of female patients. Mean age was 35 ± 14.33 years. All the samples run for anti-HCV on ELISA were also run on multi-sure rapid kit. It is evident that 22.4% were reactive on ELISA and 23.6% were reactive on rapid kit, while 75.5% were non-reactive on ELISA and 68.1% were non-reactive on rapid kit.
  • Borderline positive results were seen in 2.1% on ELISA and 5.0% on rapid kit. Sensitivity of rapid kit was 87.2%, specificity 89.3% with 82.8% positive predictive value and 98.9% negative predictive value.Multi-sure kit showed significantly, less non-reactive and more borderline results as compared to ELISA.
  • Comparison of multi-sure rapid kit with ELISA showed that core antibody can be used as an alternate marker for ELISA. Other non-structural proteins including NS3, NS4 and NS5 were found to be less significant. So, it is concluded that this rapid kit may not be recommended as an alternative of ELISA, except for places where ELISA is not available.

Monitoring of infliximab trough levels and anti-infliximab antibodies in inflammatory bowel diseases: A comparison of three commercially available ELISA kits.

There are many studies presenting data of biologics and several ELISA kits commercially available for monitoring infliximab serum trough levels (s-IFXt) and anti-drug antibodies (ADAb). We propose to compare technical characteristics and results of three different assays on a cohort of 35 patients under infliximab (IFX) and suffering from inflammatory bowel disease (IBD).s-IFXt and ADAb were systematically measured with three ELISA kits: Lisa-Tracker® Duo infliximab (Theradiag®), Ridascreen® IFX Monitoring (R-Biopharm AG®) and Promonitor® IFX (Progenika Biopharma SA®).

The main technical features that differed between kits for measuring s-IFXt were: (i) TNF coating, (ii) immune complexes revelation strategy and/or (iii) interference with other anti-TNFα agents. For kits measuring ADAb, they were revelation steps and unit of results.

There was an excellent mathematical correlation of s-IFXt between assays however Bland-Altman analysis denoted (i) s-IFXt were on average 48 to 69% higher in Ridascreen® than in the other two assays, and (ii) elevated s-IFXt were higher with Promonitor® compared to Lisa-Tracker®.

As a consequence, there were some substantial discrepancies between assays for classification of s-IFXt into concentration ranges. Despite unstandardized units, pairwise qualitative comparison showed a perfect agreement between the three pairs of ADAb assays.

Our data show that the evaluated assays are not quantitatively interchangeable due to substantial variations in some results that could lead, for some patients, to divergent therapeutic decisions. We remind to be cautious when comparing study results issued from different kits and recommend using the same assay for the longitudinal follow-up of IBD patients